| 產品描述： | Poziotinib(NOV120101; HM781-36B) is an irreversible HER1/2/4 inhibitor (IC50s: 3/5/23 nM). |
| 靶點： |
Apoptosis;EGFR;HER;Apoptosis;?EGFR;?HER |
| 體內研究： |
Poziotinib對EGFR磷酸化和下游信號級聯放大的關鍵組分有抑制作用,如STAT3,AKT和ERK,且對HER2擴增的胃癌細胞生長有特異性抑制效果。經激活HER2擴增的胃癌細胞中線粒體途徑,Poziotinib還可誘導G1細胞周期阻滯和細胞凋亡。此外,在HER2誘發的和HER2非擴增的胃癌細胞中,Poziotinib與化療劑有協同效果 |
| 細胞實驗： |
Viable cell growth is determined by an MTT reduction assay. Briefly, all cell lines are seeded at a density of 3 × 103 per well in 96-well culture plates and then incubated at 37 °C for 24 h. The cells are then treated with 0.001, 0.01, 0.1, or 10 μM of HM781-36B. Three days later, 50 μg of MTT are added to each well and the samples are then incubated for 4 h to reduce the dye. Next, the samples are treated with DMSO, after which the absorbance of the converted dye in the living cells is measured at a wavelength of 540 nm. Six replicate wells are used for each analysis, and at least three independent experiments are conducted. Data points shown represent the mean while bars represent the SE.(Only for Reference) |
| 參考文獻： |
1.Nam HJ, et al. Cancer Lett. 2011, 302(2), 155-165. 2.Cha MY, et al. Int J Cancer. 2012, 130(10), 2445-2454. |
| 溶解性： |
H2O:<1 mg/mL DMSO:91 mg/mL (185.2 mM) Ethanol:<1 mg/mL |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.035 ml |
10.176 ml |
20.352 ml |
| 5 mM |
0.407 ml |
2.035 ml |
4.07 ml |
| 10 mM |
0.204 ml |
1.018 ml |
2.035 ml |
| 50 mM |
0.041 ml |
0.204 ml |
0.407 ml |
|
| 注意： |
部分產品我司僅能提供部分信息，我司不保證所提供信息的權威性，僅供客戶參考交流研究之用。 |
上海工商
危險品化學品經營許可證（不帶存儲）
營業執照(三證合一)
北京